Improved multilocus sequence typing of Burkholderia pseudomallei and closely related species

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Expanded multilocus sequence typing for burkholderia species.

PCR primers targeting loci in the current Burkholderia cepacia complex multilocus sequence typing scheme were redesigned to (i) more reliably amplify these loci from B. cepacia complex species, (ii) amplify these same loci from additional Burkholderia species, and (iii) enable the use of a single primer set per locus for both amplification and DNA sequencing.

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Multilocus sequence typing of pathogenic Candida species.

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Australian and Thai isolates of Burkholderia pseudomallei are distinct by multilocus sequence typing: revision of a case of mistaken identity.

A recent study using multilocus sequence typing (MLST) of Burkholderia pseudomallei isolates found a sequence type (ST60) to be common to both Thailand and Australia, contradicting earlier studies showing complete distinction between isolates from these regions. The ST60 isolates reportedly from Australia had been obtained for MLST from United Kingdom and U.S. collections. We have located and c...

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Multilocus sequence typing scheme that provides both species and strain differentiation for the Burkholderia cepacia complex.

A single multilocus sequence typing (MLST) scheme was developed for precise characterization of the opportunistic pathogens of Burkholderia cepacia complex (BCC), a group composed of at least nine closely related species. Seven conserved housekeeping genes were selected after a comparison of five Burkholderia species, and a collection of strains was subjected to nucleotide sequence analysis usi...

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ژورنال

عنوان ژورنال: Journal of Medical Microbiology

سال: 2016

ISSN: 0022-2615,1473-5644

DOI: 10.1099/jmm.0.000312